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Antheridial
Development from the Isolated Protoplast
of Young Gametophyte of Tree
Fern Cyathea contaminans
(Hook) Copel.: A Scientific Approach
to Teaching about Spermatogenesis
Rodolfo S. Treyes,
Toshiyuki Kawakami & Hideo Ikeda
Faculty of Education,
Hiroshima University,
Higashi-Hiroshima,
739-8523
Japan
IntroductionMaterials
& MethodsResultsDiscussion
ReferencesAppendix
Abstract
In current biological studies, cell culture methods have been widely used for in-vitro investigation of many biological processes such as cell differentiation. In the present study, the young gametophyte of tree fern Cyathea contaminans (Hook) Copel. has been successfully utilized for isolating the antheridia primordial cell. Using the cell culture method, differentiation of antheridium from the isolated protoplasts of antheridia primordial cells was followed. Prothallial tissues comprising antheridial primordial cells were excised from the young gametophyte of the tree fern. The excised tissues were subjected to enzymatic digestion. The treatment resulted in the separation of the protoplasts from the cell wall. When the protoplasts were cultured in 1/2 strength MS medium with 0.005 mM gibberellin (GA7), 0.6 M mannitol, 0.05 M sucrose, they began to divide within the 7-day culture period. The antheridial development was generated in the presence of gibberellin (GA7) in the culture media. The antheridia containing spermatocytes completely developed approximately two weeks after the protoplast culture. The procedures used in this investigation can be applied in morphological studies of fern reproductive cells. The methods will provide the students the opportunity to manipulate cells and to observe tissue differentiation in multicellular organisms.
IntroductionMaterials
& MethodsResultsDiscussion
ReferencesAppendix